| Model Animals and Cell Lines | |
|---|---|
| Mouse xenograft tumor growth and treatment by intraperitoneal injection and Oral Gavage | ESCC, colon, lung, pancreatic, breast, thyroid normal and cancer cell lines, drosophila S2 cell line. |
| Immunization injection (rabbit, mouse) | Human primary GBM cell lines |
| Transgene fly lines (site specific gene targeting) | Established radioresistant cancer cell lines |
| Cell Biology | |
| Cell culture and transfection | Cell proliferation assay |
| Lentivirus production and target cell transduction | Colony formation assay |
| Cell culture surface coating | Cell invasion assay |
| Cell cycle analysis by flow cytometry | Foci formation assay/immunofluorescent |
| Matrigel 3D cell culture | Comet assay |
| Cell viability assay | |
| Biochemistry and Molecular Bilogy | |
| Cloning and sub-cloning | Luciferase reporter assay |
| In-fusion advantage PCR cloning | Home-made PCR protocol to test mycoplasma contamination of cell |
| Genomic DNA extraction from cell and tissue | Mitochondrial membrane potential (JC1) |
| Southern blot | Mitochondrial ROS assay |
| Protein extraction and fractionation of culture cells | Seahorse assay |
| Mitochondrial isolation from cell culture and tissue | Oxygen consumption assay |
| Western blot and protein gel staining | ATP assay |
| Immunoprecipitation | ADP/ATP ratio assay |
| Protein pull-down assay | GSH/GSSG ratio assay |
| RNA extraction from cell and tissue, qRT-PCR | Glucose reporter assay |
| RNA pull-down assay | Glucose uptake and lactate production assay |
| ChIP-PCR | Mass Spec of Metabolomics |
| Immunohistochemistry (IHC) | |
| Drosophila Genetics | |
| Establish transgene fly lines (targeted gene mutation) | RNA in situ hybridization in Drosophila Embryo |
| GAL4-UAS reporter system | Confocal imaging |
| RNA Protein double staining in Drosophila Embryo | Bioinformatics: Flybase, BLAT, EvoPrinter |
| Immunology | |
| Polyclonal antibody production: intraperitoneal (IP) injection of purified human protein into rabbit and collection of blood of immunized animals. | |
| Monoclonal antibody production: i.p. injection of synthesized small peptide and isolation of spleen cells from immunized mice. | |
| Fusion of spleen cells with immortal myeloma cells | |
| Culture of hybridoma cells | |
| Enzyme-linked immunosorbent assay (ELISA) | |
